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Sample GSM3359645 Query DataSets for GSM3359645
Status Public on Aug 31, 2019
Title human normal blood miRNA 2
Sample type RNA
Source name human normal blood miRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 40
gender: male
tissue: blood
concentration of hemoglobin (g/l): 158
wbc (×10^9/l): 9.25
platelets (×10^9/l): 37
lymphocytes (×10^9/l): 3.12
lymphocyte (%): 33.7
neutrophils (×10^9/l): 5.22
neutrophils (%): 56.5
monocytess (×10^9/l): 0.74
monocyte (%): 8
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description miRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
Platform ID GPL19117
Series (2)
GSE119151 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [miRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
VALUE RMA signal intensity

Data table
14q0_st 0.235468019
14qI-1_st 0.772477741
14qI-1_x_st 0.338166804
14qI-2_st -0.147937607
14qI-3_x_st 0.107286247
14qI-4_st 0.020184615
14qI-4_x_st 0.139437486
14qI-5_st 0.142269885
14qI-6_st 0.571786682
14qI-7_st 0.561857933
14qI-8_st -0.133630875
14qI-8_x_st -0.094505503
14qI-9_x_st -0.255876694
14qII-10_st 0.25109086
14qII-11_st 0.386775142
14qII-12_st 0.109377918
14qII-12_x_st 0.234769453
14qII-13_st 0.436535161
14qII-14_st 0.039783296
14qII-14_x_st 0.104863496

Total number of rows: 36353

Table truncated, full table size 984 Kbytes.

Supplementary file Size Download File type/resource
GSM3359645_normal2_miRNA-4_0_.CEL.gz 714.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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