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Sample GSM3359640 Query DataSets for GSM3359640
Status Public on Aug 31, 2019
Title human patient blood mRNA 3
Sample type RNA
Source name human patient blood mRNA
Organism Homo sapiens
Characteristics subject status: Chinese imported falciparum malaria patient
age (years): 32
gender: male
tissue: blood
parasitemia (%): 1.5%
concentration of hemoglobin (g/l): 176
wbc (×10^9/l): 4.12
platelets (×10^9/l): 73
lymphocytes (×10^9/l): 0.67
lymphocyte (%): 16.3
neutrophils (×10^9/l): 2.84
neutrophils (%): 69
monocytess (×10^9/l): 0.51
monocyte (%): 12.4
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description mRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
Platform ID GPL15207
Series (2)
GSE119150 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [mRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
VALUE RMA signal intensity

Data table
11715100_at 2.196307873
11715101_s_at 4.897111747
11715102_x_at 2.88721257
11715103_x_at 2.970769284
11715104_s_at 2.639592487
11715105_at 1.866826512
11715106_x_at 1.934750993
11715107_s_at 4.03769666
11715108_x_at 1.618356256
11715109_at 1.765940283
11715110_at 5.181842404
11715111_s_at 7.922593578
11715112_at 1.729490813
11715113_x_at 5.25626317
11715114_x_at 5.994572933
11715115_s_at 1.35587789
11715116_s_at 2.834532688
11715117_x_at 1.444020884
11715118_s_at 2.293470676
11715119_s_at 2.199166611

Total number of rows: 49395

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM3359640_patient3_PrimeView_.CEL.gz 1.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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