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Sample GSM3359637 Query DataSets for GSM3359637
Status Public on Aug 31, 2019
Title human normal blood mRNA 6
Sample type RNA
Source name human normal blood mRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 36
gender: male
tissue: blood
concentration of hemoglobin (g/l): 146
wbc (×10^9/l): 7.43
platelets (×10^9/l): 216
lymphocytes (×10^9/l): 2.28
lymphocyte (%): 30.7
neutrophils (×10^9/l): 4.66
neutrophils (%): 62.7
monocytess (×10^9/l): 0.39
monocyte (%): 5.2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description mRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
Platform ID GPL15207
Series (2)
GSE119150 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [mRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
VALUE RMA signal intensity

Data table
11715100_at 2.406521041
11715101_s_at 4.562554422
11715102_x_at 2.823187294
11715103_x_at 3.253909127
11715104_s_at 2.639226478
11715105_at 1.617732731
11715106_x_at 1.833364327
11715107_s_at 3.84585195
11715108_x_at 1.417598089
11715109_at 1.806072868
11715110_at 5.338341455
11715111_s_at 7.280423539
11715112_at 1.486581379
11715113_x_at 5.196234251
11715114_x_at 5.194656766
11715115_s_at 1.240747515
11715116_s_at 2.253118073
11715117_x_at 1.274010929
11715118_s_at 2.730646169
11715119_s_at 1.868516242

Total number of rows: 49395

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM3359637_normal6_PrimeView_.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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