NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3359636 Query DataSets for GSM3359636
Status Public on Aug 31, 2019
Title human normal blood mRNA 5
Sample type RNA
 
Source name human normal blood mRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 27
gender: male
tissue: blood
concentration of hemoglobin (g/l): 149
wbc (×10^9/l): 7.16
platelets (×10^9/l): 245
lymphocytes (×10^9/l): 1.91
lymphocyte (%): 26.7
neutrophils (×10^9/l): 4.48
neutrophils (%): 62.6
monocytess (×10^9/l): 0.6
monocyte (%): 8.4
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description mRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
E-mail(s) zouyang.bfh@outlook.com
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
 
Platform ID GPL15207
Series (2)
GSE119150 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [mRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
11715100_at 2.164024681
11715101_s_at 4.795311462
11715102_x_at 2.536680992
11715103_x_at 3.542225046
11715104_s_at 2.629739714
11715105_at 1.879629561
11715106_x_at 1.537212426
11715107_s_at 3.661053019
11715108_x_at 1.666236077
11715109_at 1.407119089
11715110_at 5.267701526
11715111_s_at 7.080389538
11715112_at 1.561663391
11715113_x_at 4.96412838
11715114_x_at 5.116913421
11715115_s_at 1.160644566
11715116_s_at 2.102806184
11715117_x_at 1.165946969
11715118_s_at 2.465947559
11715119_s_at 1.879629561

Total number of rows: 49395

Table truncated, full table size 1225 Kbytes.




Supplementary file Size Download File type/resource
GSM3359636_normal5_PrimeView_.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap