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Sample GSM3359635 Query DataSets for GSM3359635
Status Public on Aug 31, 2019
Title human normal blood mRNA 4
Sample type RNA
Source name human normal blood mRNA
Organism Homo sapiens
Characteristics subject status: normal, healthy subject
age (years): 22
gender: male
tissue: blood
concentration of hemoglobin (g/l): 127
wbc (×10^9/l): 7.09
platelets (×10^9/l): 207
lymphocytes (×10^9/l): 2.69
lymphocyte (%): 37.9
neutrophils (×10^9/l): 3.54
neutrophils (%): 49.9
monocytess (×10^9/l): 0.8
monocyte (%): 11.3
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description mRNA expression
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Aug 28, 2018
Last update date Sep 01, 2019
Contact name Yang Zou
Organization name Beijing Friendship Hospital Affiliated to the Capital University of Medical Sciences.
Street address Xicheng District road 95 Yongan
City beijing
ZIP/Postal code 100000
Country China
Platform ID GPL15207
Series (2)
GSE119150 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling [mRNA]
GSE119152 Whole Blood MicroRNA Biomarkers for Blood-stage of Imported Falciparum Malaria through Integrated mRNA and miRNA Expression Profiling

Data table header descriptions
VALUE RMA signal intensity

Data table
11715100_at 2.024653917
11715101_s_at 4.077495175
11715102_x_at 2.214764114
11715103_x_at 3.543260343
11715104_s_at 2.372470708
11715105_at 1.443983626
11715106_x_at 1.746801555
11715107_s_at 3.390683927
11715108_x_at 1.523345263
11715109_at 1.30575351
11715110_at 5.273540724
11715111_s_at 6.493741898
11715112_at 1.523345263
11715113_x_at 4.652513736
11715114_x_at 4.341087056
11715115_s_at 1.283990085
11715116_s_at 2.014435931
11715117_x_at 0.990593387
11715118_s_at 2.019128786
11715119_s_at 1.613425505

Total number of rows: 49395

Table truncated, full table size 1226 Kbytes.

Supplementary file Size Download File type/resource
GSM3359635_normal4_PrimeView_.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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