|
| Status |
Public on Aug 25, 2018 |
| Title |
Panc10.5 |
| Sample type |
SRA |
| |
|
| Source name |
pancreatic cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Panc10.5
cell type: pancreatic adenocarcinoma
ethnicity source: Caucasian
ip antibody: none
molecule subtype: miRNA
|
| Treatment protocol |
methlated micro RNA IP were paefformed using m6A antibody(MBL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
High Pure miRNA Isolation Kit (Roche)
RNA libraries were prepared for sequencing using TruSeq Stranded Total RNA with Ribo-Zero Kit (Illumina)
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sample 2
|
| Data processing |
Illumina Casava1.8.2 software used for basecalling.
Reads were aligned to human mature miRNA extracted from mature.fa in miRBase using CLC genomics workbench 9.0.
Data were quantified by number of mapped reads.
Genome_build: human mature miRNA extracted from mature.fa in miRBase
Supplementary_files_format_and_content: quantified data of each sample.
|
| |
|
| Submission date |
Aug 24, 2018 |
| Last update date |
Aug 27, 2018 |
| Contact name |
Ayumu Asai |
| E-mail(s) |
aasai@cfs.med.osaka-u.ac.jp
|
| Organization name |
Osaka University
|
| Street address |
2-2 Yamadaoka
|
| City |
Suita |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE119014 |
Methylated microRNAs in Pancreatic cancer cell lines |
|
| Relations |
| BioSample |
SAMN09909601 |
| SRA |
SRX4607432 |
