|
| Status |
Public on Aug 25, 2018 |
| Title |
Sample 4_miR17-native |
| Sample type |
SRA |
| |
|
| Source name |
HCT116_native microRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line background: HCT116
cell type: colorectal carcinoma
transfection: miR17-native
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isogen (Nippon gene)
RNA libraries were prepared for sequencing using TruSeq Stranded Total RNA with Ribo-Zero Kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina Casava1.8.2 software used for basecalling.
Reads were aligned to hg19 genome using CLC genomics workbench 9.0
Data were quantified by TPM.
Genome_build: hg19
Supplementary_files_format_and_content: quantified data of each sample.
|
| |
|
| Submission date |
Aug 24, 2018 |
| Last update date |
Aug 27, 2018 |
| Contact name |
Ayumu Asai |
| E-mail(s) |
aasai@cfs.med.osaka-u.ac.jp
|
| Organization name |
Osaka University
|
| Street address |
2-2 Yamadaoka
|
| City |
Suita |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE119013 |
Effect for target mRNA degradation by methylated microRNAs |
|
| Relations |
| BioSample |
SAMN09909592 |
| SRA |
SRX4607407 |
