|
| Status |
Public on Aug 16, 2018 |
| Title |
Post LPS (time 4h, BC1215 treated) 2 |
| Sample type |
SRA |
| |
|
| Source name |
Lung parenchymal homogenate
|
| Organism |
Homo sapiens |
| Characteristics |
group: Post LPS Intervention
|
| Treatment protocol |
For LPS injury, E. coli 055:B5 endotoxin was diluted in 20ml sterile PBS at 5 mg/kg ideal body weight for the donor and infused into the pulmonary arterial limb of the circuit over 5 min via automated syringe pump at T=1 hour. BC1215 was dissolved in 50ml sterile PBS and BC1215 or PBS alone was administered via a bronchoscope with 2-3 ml of solution directed into each segmental bronchus immediately following T-2 hour BAL and biopsy collection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was placed in RNA later, then RNA extracted using Qiazol
TuruSeq stranded mRNA (PolyA+)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
Post_LPS_BC1215_2
|
| Data processing |
Trimmomatic (Version 0.36)
TopHat (Version 2.1.1)
Cufflinks (Version 2.2.1)
Genome_build: GRCH37
Supplementary_files_format_and_content: Cufflinks (Version 2.2.1)
|
| |
|
| Submission date |
Aug 15, 2018 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Josiah Radder |
| Organization name |
University of Pittsburgh
|
| Department |
Pulmonary, Allergy, and Critical Care Medicine
|
| Street address |
200 Lothrop St
|
| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15224 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE118566 |
Ex Vivo Lung Perfusion as a Human Platform for Preclinical Small Molecule Testing |
|
| Relations |
| BioSample |
SAMN09836871 |
| SRA |
SRX4553862 |
