|
Status |
Public on Aug 16, 2018 |
Title |
Pre LPS (time 0h) 6 |
Sample type |
SRA |
|
|
Source name |
Lung parenchymal homogenate
|
Organism |
Homo sapiens |
Characteristics |
group: Pre LPS
|
Treatment protocol |
For LPS injury, E. coli 055:B5 endotoxin was diluted in 20ml sterile PBS at 5 mg/kg ideal body weight for the donor and infused into the pulmonary arterial limb of the circuit over 5 min via automated syringe pump at T=1 hour. BC1215 was dissolved in 50ml sterile PBS and BC1215 or PBS alone was administered via a bronchoscope with 2-3 ml of solution directed into each segmental bronchus immediately following T-2 hour BAL and biopsy collection.
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissue was placed in RNA later, then RNA extracted using Qiazol TuruSeq stranded mRNA (PolyA+)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
Pre_LPS_6
|
Data processing |
Trimmomatic (Version 0.36) TopHat (Version 2.1.1) Cufflinks (Version 2.2.1) Genome_build: GRCH37 Supplementary_files_format_and_content: Cufflinks (Version 2.2.1)
|
|
|
Submission date |
Aug 15, 2018 |
Last update date |
Aug 16, 2018 |
Contact name |
Josiah Radder |
Organization name |
University of Pittsburgh
|
Department |
Pulmonary, Allergy, and Critical Care Medicine
|
Street address |
200 Lothrop St
|
City |
Pittsburgh |
State/province |
Pennsylvania |
ZIP/Postal code |
15224 |
Country |
USA |
|
|
Platform ID |
GPL21290 |
Series (1) |
GSE118566 |
Ex Vivo Lung Perfusion as a Human Platform for Preclinical Small Molecule Testing |
|
Relations |
BioSample |
SAMN09836876 |
SRA |
SRX4553857 |