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Sample GSM3319569 Query DataSets for GSM3319569
Status Public on Feb 04, 2019
Title LNCaP_EtOH_DMSO rep2
Sample type SRA
 
Source name LNCaP_EtOH_DMSO
Organism Homo sapiens
Characteristics cell line: LNCaP-FGC prostate adenocarcinoma cancer cell line
treatment: EtOH_DMSO
Treatment protocol Cells were pretreated with indicated inhibitors for 45 minutes followed by 24 hr stimulation with 5 nM DHT or EtOH.
Growth protocol LNCaP-FGC cells were grown for 72 hrs in charcoal stripped (steroid free) serum,
Extracted molecule total RNA
Extraction protocol RNA was prepared by Qiazol extraction followed by RNeasy mini column (QIAGEN)
10 ug of total RNA was submitted to poly-A selection to remove ribosomal RNA and reverse transcribed to double-stranded cDNA. The cDNA was eblunt ended, adenylated, and ligated with Illumina indexes.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description Sample_2
Data processing Illumina Casava1.8 software used for basecalling
Reads were aligned using the OmicSoft aligner and gene expression quantified based on UCSC gene model.
Differentially expressed genes between treatment conditions detected by DESeq2 with a statistical cut-off (FC>2, p<0.01)
Genome_build: B37
Supplementary_files_format_and_content: FPKMs
 
Submission date Aug 06, 2018
Last update date Feb 04, 2019
Contact name Xin Lu
E-mail(s) xin.x.lu@abbvie.com
Phone 847-937-7942
Organization name Abbvie
Department Oncology Discovery
Street address 1 North Waukegan Rd
City North Chicago
State/province IL
ZIP/Postal code 60064
Country USA
 
Platform ID GPL21290
Series (2)
GSE118152 Selective Inhibition of the Second Bromodomain of BET Family Maintains Anti-Tumor Efficacy and Improves Tolerability (LNCaP RNA-seq)
GSE130269 Selective Inhibition of the Second Bromodomain of BET Family Maintains Anti-Tumor Efficacy and Improves Tolerability
Relations
BioSample SAMN09768218
SRA SRX4510394

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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