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Sample GSM3317673 Query DataSets for GSM3317673
Status Public on Dec 04, 2018
Title 32h Replicate 2
Sample type SRA
 
Source name 32h, HPAF-II xenograft - mouse pancreas
Organism Homo sapiens
Characteristics host: mouse
rin: 7
timepoint: 32h
cell type: HPAF-II
treatment: ETC-159 (PORCN inhbitor)
Treatment protocol Treatment with ETC-159 bid
Growth protocol HPAF-II cells injected into the mouse pancreas. Tumors were allowed to develop for 28 days before start of treatment
Extracted molecule polyA RNA
Extraction protocol RNAeasy kit (Qiagen)
Illumina TruSeq Stranded polyA enrichment
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description Human graft / mouse host
Data processing adaptor trimming using Cutadapt
filtering using Xenome
alignment using STAR - hg38 Ensembl 79
quantification using STAR - hg38 Ensembl 79
Genome_build: hg38 / Ensembl 79
Supplementary_files_format_and_content: RSEM quantification of human reads
 
Submission date Aug 02, 2018
Last update date Dec 04, 2018
Contact name NATHAN HARMSTON
E-mail(s) harmstonn@cardiff.ac.uk
Organization name Cardiff University
Street address Museum Avenue
City Cardiff
ZIP/Postal code CF10 3AX
Country United Kingdom
 
Platform ID GPL21290
Series (1)
GSE118041 Transcriptional response to PORCN inhibition using an orthotopic model of RNF43 mutant pancreatic adenocarcinoma (HPAF-II)
Relations
SRA SRX3875377
BioSample SAMN08848855

Supplementary file Size Download File type/resource
GSM3317673_nph_m14-32h.genes.results.txt.gz 1.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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