|
Status |
Public on Dec 04, 2018 |
Title |
8h Replicate 4 |
Sample type |
SRA |
|
|
Source name |
8h, HPAF-II xenograft - mouse pancreas
|
Organism |
Homo sapiens |
Characteristics |
host: mouse rin: 6.2 timepoint: 8h cell type: HPAF-II treatment: ETC-159 (PORCN inhbitor)
|
Treatment protocol |
Treatment with ETC-159 bid
|
Growth protocol |
HPAF-II cells injected into the mouse pancreas. Tumors were allowed to develop for 28 days before start of treatment
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNAeasy kit (Qiagen) Illumina TruSeq Stranded polyA enrichment
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
Human graft / mouse host
|
Data processing |
adaptor trimming using Cutadapt filtering using Xenome alignment using STAR - hg38 Ensembl 79 quantification using STAR - hg38 Ensembl 79 Genome_build: hg38 / Ensembl 79 Supplementary_files_format_and_content: RSEM quantification of human reads
|
|
|
Submission date |
Aug 02, 2018 |
Last update date |
Dec 04, 2018 |
Contact name |
NATHAN HARMSTON |
E-mail(s) |
harmstonn@cardiff.ac.uk
|
Organization name |
Cardiff University
|
Street address |
Museum Avenue
|
City |
Cardiff |
ZIP/Postal code |
CF10 3AX |
Country |
United Kingdom |
|
|
Platform ID |
GPL21290 |
Series (1) |
GSE118041 |
Transcriptional response to PORCN inhibition using an orthotopic model of RNF43 mutant pancreatic adenocarcinoma (HPAF-II) |
|
Relations |
SRA |
SRX3875354 |
BioSample |
SAMN08848845 |