NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3290433 Query DataSets for GSM3290433
Status Public on Nov 12, 2020
Title A42323
Sample type RNA
 
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Extramammary paget disease scrotum
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
 
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
 
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
E-mail(s) linabu@cau.edu.cn
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
 
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
ID_REF
VALUE The normalized value of 75 percentile (log2)

Data table
ID_REF VALUE
A_33_P3211513 1371.505
A_24_P278460 1241.772
A_21_P0014651 230.373
A_23_P61637 1444.157
A_24_P120109 28.67172
A_23_P63896 3728.770408
A_24_P187056 1997.154
A_23_P122001 4066.889062
A_21_P0004300 58.29967
A_23_P88740 2406.321
A_23_P11652 10422.87093
A_23_P104025 1989.33681
A_23_P63459 10442.79
A_23_P252748 5373.397802
A_21_P0002212 343.4517
A_21_P0004440 223.1674
A_23_P31073 1864.081
A_33_P3331085 233.7621
A_23_P123727 4106.735901
A_23_P109774 3000.520284

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.




Supplementary file Size Download File type/resource
GSM3290433_US10313827_253949448652_S01_GE1_1105_Oct12_2_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap