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Sample GSM3290429 Query DataSets for GSM3290429
Status Public on Nov 12, 2020
Title A42537
Sample type RNA
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Normal skin
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
VALUE The normalized value of 75 percentile (log2)

Data table
A_33_P3211513 366.5899
A_24_P278460 429.5433
A_21_P0014651 19.05846
A_23_P61637 178.8946
A_24_P120109 13.22867
A_23_P63896 405.1196286
A_24_P187056 571.4217
A_23_P122001 1339.831953
A_21_P0004300 8.053402
A_23_P88740 247.0648
A_23_P11652 3346.618942
A_23_P104025 319.7817452
A_23_P63459 1203.782
A_23_P252748 1195.31826
A_21_P0002212 74.00711
A_21_P0004440 45.60643
A_23_P31073 960.3781
A_33_P3331085 72.28418
A_23_P123727 1587.167721
A_23_P109774 433.9840977

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.

Supplementary file Size Download File type/resource
GSM3290429_US10313827_253949448652_S01_GE1_1105_Oct12_1_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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