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Sample GSM3290428 Query DataSets for GSM3290428
Status Public on Nov 12, 2020
Title A42345
Sample type RNA
 
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Normal skin
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
 
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
 
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
E-mail(s) linabu@cau.edu.cn
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
 
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
ID_REF
VALUE The normalized value of 75 percentile (log2)

Data table
ID_REF VALUE
A_33_P3211513 424.0318
A_24_P278460 318.1819
A_21_P0014651 20.26622
A_23_P61637 131.1064
A_24_P120109 11.74109
A_23_P63896 487.5727415
A_24_P187056 689.2234
A_23_P122001 1360.815194
A_21_P0004300 17.15359
A_23_P88740 511.3666
A_23_P11652 3286.471083
A_23_P104025 575.6074242
A_23_P63459 2123.519
A_23_P252748 1120.718466
A_21_P0002212 81.05933
A_21_P0004440 74.22248
A_23_P31073 879.1892
A_33_P3331085 138.5864
A_23_P123727 1248.968874
A_23_P109774 418.914955

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.




Supplementary file Size Download File type/resource
GSM3290428_US10313827_253949448652_S01_GE1_1105_Oct12_1_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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