GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM3290427 Query DataSets for GSM3290427
Status Public on Nov 12, 2020
Title A41785
Sample type RNA
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Normal skin
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
VALUE The normalized value of 75 percentile (log2)

Data table
A_33_P3211513 454.0197
A_24_P278460 236.7815
A_21_P0014651 52.35798
A_23_P61637 473.0328
A_24_P120109 4.318427
A_23_P63896 586.9357832
A_24_P187056 215.7098
A_23_P122001 1027.973181
A_21_P0004300 4.602187
A_23_P88740 769.2572
A_23_P11652 2732.717402
A_23_P104025 513.651296
A_23_P63459 2509.755
A_23_P252748 872.8018143
A_21_P0002212 72.06483
A_21_P0004440 40.9678
A_23_P31073 532.979
A_33_P3331085 108.5884
A_23_P123727 693.2182028
A_23_P109774 350.0054276

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.

Supplementary file Size Download File type/resource
GSM3290427_US10313827_253949448652_S01_GE1_1105_Oct12_1_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap