NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3262713 Query DataSets for GSM3262713
Status Public on Sep 23, 2018
Title ILC2_RNA
Sample type SRA
 
Source name Five-day cultured ILC2 cells generated from primary splenic Lin-CD127hiThy1.2+TCRb-ST2hi cells
Organism Mus musculus
Characteristics cell type: ILC2
strain: BALB/c
tissue: ILC2 cells
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description Splenic ILC2 cells were culture under conditions (10 U/ml IL-7, 10 ng/ml IL-25, 10ng/ml IL-33) for 5 days in vitro.
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
 
Submission date Jul 10, 2018
Last update date Sep 24, 2018
Contact name Yusuke ENDO
E-mail(s) san3tamariayuyu@chiba-u.jp
Phone 81432262186
Organization name Chiba University
Department Immunology
Lab Immunology
Street address 1-8-1 Inohana, Chuo-ku
City Chiba-city
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL18480
Series (1)
GSE116842 DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells
Relations
BioSample SAMN09635574
SRA SRX4372642

Supplementary file Size Download File type/resource
GSM3262713_ILC2_RNA.txt.gz 132.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap