|
| Status |
Public on Aug 18, 2020 |
| Title |
LnCaP_Combination_Replicate1 |
| Sample type |
SRA |
| |
|
| Source name |
LnCaP Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LnCaP
treatment: OSMI2 & AT7519 treated
|
| Treatment protocol |
LNCaP cells were treated with control, 40mM OSMI-2-only, 0.5mM AT7519-only and 0.5mM AT7519+40mM OSMI-2, for 4 hours prior to harvesting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Illustra RNAspin Minikit (GE healthcare, cat num. 25-0500-72)
Libraries for sequencing were prepared by the Norwegian High Throughput Sequencing Centre using Strand-specific TruSeq RNA-seq library prep kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Two biological replicates of each condition were used for the assay
Samples were multiplexed and paired-end sequenced with 150bp read length
Sequencing reads were aligned to Hg19 (GRCh37) using STAR
Raw counts of reads were mapped to genes using HTSeq counts
Normalised counts were generated using DESeq2
Genome_build: Hg19 (GRCh37)
|
| |
|
| Submission date |
Jul 09, 2018 |
| Last update date |
Aug 18, 2020 |
| Contact name |
Rebecca Elizabeth Steele |
| E-mail(s) |
rebecca.steele@icr.ac.uk
|
| Organization name |
Queen's University Belfast
|
| Department |
CCRCB
|
| Street address |
97 Lisburn Road
|
| City |
Belfast |
| ZIP/Postal code |
BT97AE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE116778 |
Inhibition of O-GlcNAc transferase renders prostate cancer cells dependent on CDK9 |
|
| Relations |
| BioSample |
SAMN09631065 |
| SRA |
SRX4368786 |
