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Sample GSM3261821 Query DataSets for GSM3261821
Status Public on Aug 18, 2020
Title LnCaP_Control_Replicate1
Sample type SRA
 
Source name LnCaP Cell Line
Organism Homo sapiens
Characteristics cell line: LnCaP
treatment: Control
Treatment protocol LNCaP cells were treated with control, 40mM OSMI-2-only, 0.5mM AT7519-only and 0.5mM AT7519+40mM OSMI-2, for 4 hours prior to harvesting
Extracted molecule total RNA
Extraction protocol RNA was isolated with Illustra RNAspin Minikit (GE healthcare, cat num. 25-0500-72)
Libraries for sequencing were prepared by the Norwegian High Throughput Sequencing Centre using Strand-specific TruSeq RNA-seq library prep kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing Two biological replicates of each condition were used for the assay
Samples were multiplexed and paired-end sequenced with 150bp read length
Sequencing reads were aligned to Hg19 (GRCh37) using STAR
Raw counts of reads were mapped to genes using HTSeq counts
Normalised counts were generated using DESeq2
Genome_build: Hg19 (GRCh37)
 
Submission date Jul 09, 2018
Last update date Aug 18, 2020
Contact name Rebecca Elizabeth Steele
E-mail(s) rebecca.steele@icr.ac.uk
Organization name Queen's University Belfast
Department CCRCB
Street address 97 Lisburn Road
City Belfast
ZIP/Postal code BT97AE
Country United Kingdom
 
Platform ID GPL21290
Series (1)
GSE116778 Inhibition of O-GlcNAc transferase renders prostate cancer cells dependent on CDK9
Relations
BioSample SAMN09631071
SRA SRX4368780

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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