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Sample GSM3259696 Query DataSets for GSM3259696
Status Public on Sep 01, 2018
Title US45103089_253949417625_S01_GE1_1100_36K_2_2
Sample type RNA
 
Source name FFPE
Organism Homo sapiens
Characteristics tissue: Tumor Sample, advanced rectal adenocarcinoma
age at surgery: 64
dworak tumor regression grade: 4
gender: M
Treatment protocol chemoradiation therapy, a combination of capecitabine (825 mg/m2) and radiations (50.4 Gy) provided in 28 fractions in 12 weeks. Clinical staging was accomplished using two physical examinations, endoscopic ultrasound (EUS) and nuclear magnetic resonance (NMR) before the administration of the neoadjuvant treatment. After 8 weeks from the completion of neoadjuvant therapy, surgery was performed
Extracted molecule total RNA
Extraction protocol RNA extraction
Label Cy3
Label protocol Agilent SureTag Labeling kit (Agilent Technologies, Palo Alto, CA, USA), enzymatically label 1.8 µg of amplified cDNA with Cyanine 3-dUTP for 2 hours at 37°C
 
Hybridization protocol Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray.
Scan protocol Slides were scanned (Agilent SureScan Microarray Scanner) immediately after washing procedure according to the precedure outlined in Agilent one-color Microarray-based gene expression analysis manual.
Description Gene Expression after capecitabine and radiotherapy cycle of 8 weeks
Data processing The scanned images were analyzed with Feature Extraction Software v10.7 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jul 06, 2018
Last update date Sep 01, 2018
Contact name Lorenzo Ferrando
E-mail(s) lorenzo.ferrando@edu.unige.it
Organization name IRCCS - Ospedale Policlinico San Martino
Department Department of Internal Medicine
Lab Translational Laboratory
Street address Viale Benedetto XV
City Genova
ZIP/Postal code 16132
Country Italy
 
Platform ID GPL17077
Series (1)
GSE116742 Analysis of lncRNAs for the characterization of the response to neoadjuvant chemoradiotherapy

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_23_P117082 11.58276915
A_33_P3246448 8.176522099
A_33_P3318220 10.52664103
A_33_P3236322 13.87113711
A_33_P3319925 10.37679824
A_21_P0000509 15.46709667
A_21_P0000744 7.43708598
A_24_P215804 7.464096196
A_23_P110167 13.13916007
A_33_P3211513 11.38722034
A_23_P103349 8.459331936
A_32_P61480 5.611431281
A_33_P3788124 8.305582856
A_33_P3414202 12.36392574
A_33_P3316686 10.07229338
A_33_P3300975 9.25749385
A_33_P3263061 11.29108145
A_33_P3261373 11.20524545
A_24_P278460 8.787641318
A_21_P0013109 8.420254881

Total number of rows: 50599

Table truncated, full table size 1255 Kbytes.




Supplementary file Size Download File type/resource
GSM3259696_US45103089_253949417625_S01_GE1_1100_36K_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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