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Sample GSM3259691 Query DataSets for GSM3259691
Status Public on Sep 01, 2018
Title US45103089_253949417607_S01_GE1_1100_25K_1_1
Sample type RNA
 
Source name FFPE
Organism Homo sapiens
Characteristics tissue: Tumor Sample, advanced rectal adenocarcinoma
age at surgery: 68
dworak tumor regression grade: 4
gender: F
Treatment protocol chemoradiation therapy, a combination of capecitabine (825 mg/m2) and radiations (50.4 Gy) provided in 28 fractions in 12 weeks. Clinical staging was accomplished using two physical examinations, endoscopic ultrasound (EUS) and nuclear magnetic resonance (NMR) before the administration of the neoadjuvant treatment. After 8 weeks from the completion of neoadjuvant therapy, surgery was performed
Extracted molecule total RNA
Extraction protocol RNA extraction
Label Cy3
Label protocol Agilent SureTag Labeling kit (Agilent Technologies, Palo Alto, CA, USA), enzymatically label 1.8 µg of amplified cDNA with Cyanine 3-dUTP for 2 hours at 37°C
 
Hybridization protocol Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray.
Scan protocol Slides were scanned (Agilent SureScan Microarray Scanner) immediately after washing procedure according to the precedure outlined in Agilent one-color Microarray-based gene expression analysis manual.
Description Gene Expression after capecitabine and radiotherapy cycle of 8 weeks
Data processing The scanned images were analyzed with Feature Extraction Software v10.7 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jul 06, 2018
Last update date Sep 01, 2018
Contact name Lorenzo Ferrando
E-mail(s) lorenzo.ferrando@edu.unige.it
Organization name IRCCS - Ospedale Policlinico San Martino
Department Department of Internal Medicine
Lab Translational Laboratory
Street address Viale Benedetto XV
City Genova
ZIP/Postal code 16132
Country Italy
 
Platform ID GPL17077
Series (1)
GSE116742 Analysis of lncRNAs for the characterization of the response to neoadjuvant chemoradiotherapy

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_23_P117082 12.99954982
A_33_P3246448 8.561777877
A_33_P3318220 7.895847713
A_33_P3236322 11.56597234
A_33_P3319925 7.217101498
A_21_P0000509 13.71193077
A_21_P0000744 8.617020367
A_24_P215804 10.12334973
A_23_P110167 13.6661296
A_33_P3211513 11.34639552
A_23_P103349 9.887717394
A_32_P61480 7.388906795
A_33_P3788124 7.682374106
A_33_P3414202 11.24277415
A_33_P3316686 9.82172611
A_33_P3300975 11.17829343
A_33_P3263061 10.90467474
A_33_P3261373 8.228113058
A_24_P278460 8.35778644
A_21_P0013109 7.810592866

Total number of rows: 50599

Table truncated, full table size 1256 Kbytes.




Supplementary file Size Download File type/resource
GSM3259691_US45103089_253949417607_S01_GE1_1100_25K_1_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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