|
| Status |
Public on Jul 13, 2011 |
| Title |
016558 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Patient Cal after Etanercept treatment
|
| Organism |
Homo sapiens |
| Characteristics |
Sample_reference: Patient Cal after Etanercept treatment
Subject_reference: Cal
Tissue: mononuclear blood cells
RNA_quality: null
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
| |
|
| Channel 2 |
| Source name |
Patient Cal before Etanercept treatment
|
| Organism |
Homo sapiens |
| Characteristics |
Sample_reference: Patient Cal before Etanercept treatment
Subject_reference: Cal
Tissue: mononuclear blood cells
RNA_quality: null
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
| |
|
| |
| Hybridization protocol |
Agilent : 3000.0 ng at 60 degree_C during 17 hours
|
| Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner.
Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
| Description |
Patient Cal after Etanercept treatment vs Patient Cal before Etanercept treatment
Date:2006/08/31
|
| Data processing |
standard Lowess normalization method with selection of spots presenting a signal > 3SNR
|
| |
|
| Submission date |
Sep 23, 2008 |
| Last update date |
Jul 13, 2011 |
| Contact name |
emmanuelle meugnier |
| E-mail(s) |
emmanuelle.meugnier@univ-lyon1.fr
|
| Organization name |
INSERMU1060/INRAE1397
|
| Lab |
CarMeN
|
| Street address |
Cens Eli Bat 2 D- Hopital Lyon Sud
|
| City |
Pierre Bénite |
| ZIP/Postal code |
69495 |
| Country |
France |
| |
|
| Platform ID |
GPL1456 |
| Series (1) |
| GSE12897 |
Gene expression profiling in peripheral blood cells of patients with rheumatoid arthitis in response of anti-TNF-alpha |
|
