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Sample GSM323385 Query DataSets for GSM323385
Status Public on Jul 13, 2011
Title 016469
Sample type RNA
 
Channel 1
Source name Patient Cov after Adalimumab treatment
Organism Homo sapiens
Characteristics Sample_reference: Patient Cov after Adalimumab treatment
Subject_reference: Cov
Tissue: mononuclear blood cells
RNA_quality: null
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy3
Label protocol Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
 
Channel 2
Source name Patient Cov before Adalimumab treatment
Organism Homo sapiens
Characteristics Sample_reference: Patient Cov before Adalimumab treatment
Subject_reference: Cov
Tissue: mononuclear blood cells
RNA_quality: null
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy5
Label protocol Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
 
 
Hybridization protocol Agilent : 3000.0 ng at 60 degree_C during 17 hours
Scan protocol Scanned on an GenePix 4000B fluorescent scanner.
Image intensity data were extracted with GenePix Pro 6.0 analysis software.
Description Patient Cov after Adalimumab treatment vs Patient Cov before Adalimumab treatment
Date:2006/07/21
Data processing standard Lowess normalization method with selection of spots presenting a signal > 3SNR
 
Submission date Sep 23, 2008
Last update date Jul 13, 2011
Contact name emmanuelle meugnier
E-mail(s) emmanuelle.meugnier@univ-lyon1.fr
Organization name INSERMU1060/INRAE1397
Lab CarMeN
Street address Cens Eli Bat 2 D- Hopital Lyon Sud
City Pierre Bénite
ZIP/Postal code 69495
Country France
 
Platform ID GPL1456
Series (1)
GSE12897 Gene expression profiling in peripheral blood cells of patients with rheumatoid arthitis in response of anti-TNF-alpha

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (635/532) of the means, before treatment/after treatment

Data table
ID_REF VALUE
1 0.628616342
2
3 0.501503045
4
5 0.691416013
6 0.467290627
7 0.545285881
8 0.54819924
9 1.061464275
10 0.326156968
11 0.08711788
12 -0.310183802
13 0.36904324
14 0.17244282
15 0.555761896
16 0.509812425
17 0.037581111
18 0.358119665
19 0.564118905
20 0.709779217

Total number of rows: 27648

Table truncated, full table size 424 Kbytes.




Supplementary file Size Download File type/resource
GSM323385.gpr.gz 2.1 Mb (ftp)(http) GPR
Processed data included within Sample table

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