|
Status |
Public on Jul 13, 2011 |
Title |
016469 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Patient Cov after Adalimumab treatment
|
Organism |
Homo sapiens |
Characteristics |
Sample_reference: Patient Cov after Adalimumab treatment Subject_reference: Cov Tissue: mononuclear blood cells RNA_quality: null
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
|
|
Channel 2 |
Source name |
Patient Cov before Adalimumab treatment
|
Organism |
Homo sapiens |
Characteristics |
Sample_reference: Patient Cov before Adalimumab treatment Subject_reference: Cov Tissue: mononuclear blood cells RNA_quality: null
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy5
|
Label protocol |
Fluorescent probes were synthesized by chemical coupling of 5µgaminoallyl aRNA with cyanine 3 (Cy3) or Cy5 dyes and purified using Rneasy Mini Kit (Qiagen)
|
|
|
|
Hybridization protocol |
Agilent : 3000.0 ng at 60 degree_C during 17 hours
|
Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
Description |
Patient Cov after Adalimumab treatment vs Patient Cov before Adalimumab treatment Date:2006/07/21
|
Data processing |
standard Lowess normalization method with selection of spots presenting a signal > 3SNR
|
|
|
Submission date |
Sep 23, 2008 |
Last update date |
Jul 13, 2011 |
Contact name |
emmanuelle meugnier |
E-mail(s) |
emmanuelle.meugnier@univ-lyon1.fr
|
Organization name |
INSERMU1060/INRAE1397
|
Lab |
CarMeN
|
Street address |
Cens Eli Bat 2 D- Hopital Lyon Sud
|
City |
Pierre Bénite |
ZIP/Postal code |
69495 |
Country |
France |
|
|
Platform ID |
GPL1456 |
Series (1) |
GSE12897 |
Gene expression profiling in peripheral blood cells of patients with rheumatoid arthitis in response of anti-TNF-alpha |
|