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Sample GSM3189775 Query DataSets for GSM3189775
Status Public on Aug 26, 2018
Title NHP_F_DPI_3
Sample type SRA
 
Source name Whole Blood
Organism Macaca mulatta
Characteristics dpi: 3
Treatment protocol Samples were inactivated with Trizol LS
Growth protocol Whole blood samples were collected from NHPs at various days post Ebola Makona infection and treated with Trizol LS at a ratio of 1:3 of blood to Trizol
Extracted molecule total RNA
Extraction protocol Extration was done following the standard Trizol protocol
Libraries were contstructed using the Illumina Ribo Zero prep kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Ebola Makona infected Non-Human Primate
Data processing Raw files were filtered for read quality and length using the fastx-toolkit
Alignments were generated using Tophat which calls Bowtie2
Counts were generated with HTSeq-count
Genome_build: Rhesus Macaque
Supplementary_files_format_and_content: Count files as a tab delimiated text file
 
Submission date Jun 13, 2018
Last update date Aug 26, 2018
Contact name Emily Speranza
E-mail(s) sperane@ccf.org
Organization name Cleveland Clinic Lerner Research Institute
Department Florida Research and Innovation Center
Street address 9801 SW Discovery Wy
City Port Saint Lucie
State/province FL
ZIP/Postal code 34987
Country USA
 
Platform ID GPL21120
Series (1)
GSE115785 Whole blood RNA-Sequencing from 12 Rhesus Macaques following Ebola Makona Infection
Relations
BioSample SAMN09423372
SRA SRX4212991

Supplementary file Size Download File type/resource
GSM3189775_NHP_F_DPI_3.txt.gz 88.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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