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Sample GSM3189772 Query DataSets for GSM3189772
Status Public on Aug 26, 2018
Title NHP_E_DPI_NEC2
Sample type SRA
 
Source name Whole Blood
Organism Macaca mulatta
Characteristics dpi: NEC
Treatment protocol Samples were inactivated with Trizol LS
Growth protocol Whole blood samples were collected from NHPs at various days post Ebola Makona infection and treated with Trizol LS at a ratio of 1:3 of blood to Trizol
Extracted molecule total RNA
Extraction protocol Extration was done following the standard Trizol protocol
Libraries were contstructed using the Illumina Ribo Zero prep kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Ebola Makona infected Non-Human Primate
Data processing Raw files were filtered for read quality and length using the fastx-toolkit
Alignments were generated using Tophat which calls Bowtie2
Counts were generated with HTSeq-count
Genome_build: Rhesus Macaque
Supplementary_files_format_and_content: Count files as a tab delimiated text file
 
Submission date Jun 13, 2018
Last update date Aug 26, 2018
Contact name Emily Speranza
E-mail(s) sperane@ccf.org
Organization name Cleveland Clinic Lerner Research Institute
Department Florida Research and Innovation Center
Street address 9801 SW Discovery Wy
City Port Saint Lucie
State/province FL
ZIP/Postal code 34987
Country USA
 
Platform ID GPL21120
Series (1)
GSE115785 Whole blood RNA-Sequencing from 12 Rhesus Macaques following Ebola Makona Infection
Relations
BioSample SAMN09423410
SRA SRX4212988

Supplementary file Size Download File type/resource
GSM3189772_NHP_E_DPI_NEC2.txt.gz 96.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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