NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3187506 Query DataSets for GSM3187506
Status Public on Jul 15, 2020
Title GXA_3044 Type: Patient derived Xenograft Passage: 4
Sample type genomic
 
Source name patient-derived tumor xenograft
Organism Homo sapiens
Characteristics passages: 4
Treatment protocol none
Growth protocol PDX were passaged from mouse to mouse until DNA extraction
Extracted molecule genomic DNA
Extraction protocol DNA was extracted from snap frozen tumor xenografts, patient tumors or normal tissues. Samples were digested with proteinase K at 55°C overnight and the lysate was digested with DNAse-free RNAse (Qiagen). DNA was extracted with a mix of phenol:chloroform:isoamylalcohol and precipitated with ethanol. DNA pellets were washed and resuspended in Tris-EDTA buffer. Each DNA preparation was subjected to quality control using a 1.3% agarose gel and using NanoDrop 2000 to control for purity. Only DNA samples with no sign of degradation (a clearly defined high molecular weight band on the agarose gel) and the DNA was pure (260/280 nm and 260/230 nm close to 2.0), were used for the microarray analysis.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Data processing Affymetrix Genome wide SNP6.0 CEL files were loaded into the Affymetrix Genotyping Console 4.1 Software (Affymetrix). After scanning, arrays were checked for quality using the Affymetrix Genotyping Console. In accordance with Affymetrix guidelines, SNP6.0 arrays with a Contrast QC value above 0.4 and MAPD below 0.35 were excluded from further analysis. Copy number variations were calculated using Affymetrix GTC v4.1 and PICNIC software provided by the Cancer Genome Project from the Welcome Trust Sanger Institute. The PICNIC algorithm (PICNIC - Predicting Integral Copy Numbers In Cancer) includes improved normalisation of the data together with determination of underlying copy numbers for each segment by genome wide analysis of allele ratio and signal strength data. The data was subsequently rescaled and plotted onto its predicted underlying integer value and segmentation was applied. This data analysis procedure also allowed for assignment of a genotype to each SNP. The PICNIC segment files were further compiled to generate a simplified gene matrix. A gene was assigned a deletion (CNV=0) if a deletion segment overlaps with the gene, otherwise the copy number of the most amplified segment overlapping with the gene was assigned.
PICNIC integer copy number score (0 is considered as a gene deletion, values between 1 and 7 are considered as a moderate gene copy alteration, values equal or greater than 8 are considered as a gene amplification)
 
Submission date Jun 12, 2018
Last update date Jul 15, 2020
Contact name Vincent Vuaroqueaux
E-mail(s) vincent.vuaroqueaux@4hf.eu
Organization name 4HF Biotec
Department Bioinformatics
Street address Am Flughafen 14
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platform ID GPL6801
Series (2)
GSE115674 Genomic data from 48 Asian gastric patient-derived xenograft (PDX) models, 7 Asian gastric patient tumors and the 8 corresponding normal tissues
GSE115755 Establishment of patient-derived tumor xenografts from Asian gastric adenocarcinoma is characterized by clonal selection and bias in molecular subtypes

Supplementary file Size Download File type/resource
GSM3187506_A2671-17.CEL.gz 27.9 Mb (ftp)(http) CEL
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap