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Sample GSM3168265 Query DataSets for GSM3168265
Status Public on May 30, 2021
Title spleen_low Se_GD_rep3
Sample type RNA
 
Source name spleen_low Se_GD
Organism Mus musculus
Characteristics strain: balb/c
Sex: female
mouse condition: Graves’ disease (GD)
dietary se supply: low
tissue: spleen
Extracted molecule total RNA
Extraction protocol RNA was isolated from spleen and then quantified by NanoDrop ND-1000 (NanoDrop Technologies Inc., USA). RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Whole Mouse Genome Oligo Microarrays (Agilent Technologies) were used for gene expression analyses and offer a broad view on all known genes and transcripts in the mouse genome.
Label biotin
Label protocol Sample labeling according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
 
Hybridization protocol Array hybridizations were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Scan protocol Agilent Feature Extraction software (version 11.0.1.1) was used to analyze the acquired array images.
Description Group5_50
Data processing Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software package (Agilent Technologies).
Differentially expressed genes between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed using the R scripts. Gene ontology and pathway analyses were performed in the standard enrichment computation method.
 
Submission date May 31, 2018
Last update date May 30, 2021
Contact name YuFeng Liu
E-mail(s) lyf10182@126.com
Phone 18691529650
Organization name mouse
Street address The First Affiliated Hospital of Xi'an Jiao Tong University
City Xi'an
State/province ShanXi
ZIP/Postal code 710000
Country China
 
Platform ID GPL11202
Series (1)
GSE115152 Role of Selenium Intake for Risk and Development of Graves’ Disease

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
GE_BrightCorner 11.609948
DarkCorner 2.4041312
A_55_P1989846 8.817909
A_55_P1991598 3.0764146
A_55_P2022211 7.727232
A_55_P1980764 7.438981
A_55_P1964375 9.382526
A_51_P128876 13.589396
A_55_P2121042 3.123707
A_52_P219230 5.050447
A_51_P207591 5.119991
A_55_P2131920 7.326513
A_55_P2404223 6.363516
A_55_P2101944 17.344374
A_52_P358860 7.466228
A_51_P119031 8.517512
A_51_P309854 2.4058847
A_51_P343900 11.113323
A_51_P234359 8.067862
A_51_P487813 9.02788

Total number of rows: 39485

Table truncated, full table size 893 Kbytes.




Supplementary file Size Download File type/resource
GSM3168265_Group5_50.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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