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Sample GSM3168264 Query DataSets for GSM3168264
Status Public on May 30, 2021
Title spleen_low Se_GD_rep2
Sample type RNA
Source name spleen_low Se_GD
Organism Mus musculus
Characteristics strain: balb/c
Sex: female
mouse condition: Graves’ disease (GD)
dietary se supply: low
tissue: spleen
Extracted molecule total RNA
Extraction protocol RNA was isolated from spleen and then quantified by NanoDrop ND-1000 (NanoDrop Technologies Inc., USA). RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Whole Mouse Genome Oligo Microarrays (Agilent Technologies) were used for gene expression analyses and offer a broad view on all known genes and transcripts in the mouse genome.
Label biotin
Label protocol Sample labeling according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Hybridization protocol Array hybridizations were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Scan protocol Agilent Feature Extraction software (version was used to analyze the acquired array images.
Description Group5_31
Data processing Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software package (Agilent Technologies).
Differentially expressed genes between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed using the R scripts. Gene ontology and pathway analyses were performed in the standard enrichment computation method.
Submission date May 31, 2018
Last update date May 30, 2021
Contact name YuFeng Liu
Phone 18691529650
Organization name mouse
Street address The First Affiliated Hospital of Xi'an Jiao Tong University
City Xi'an
State/province ShanXi
ZIP/Postal code 710000
Country China
Platform ID GPL11202
Series (1)
GSE115152 Role of Selenium Intake for Risk and Development of Graves’ Disease

Data table header descriptions
VALUE normalized

Data table
GE_BrightCorner 11.609948
DarkCorner 2.323937
A_55_P1989846 8.74931
A_55_P1991598 3.4101696
A_55_P2022211 8.147213
A_55_P1980764 7.8719397
A_55_P1964375 9.318446
A_51_P128876 13.617104
A_55_P2121042 3.3612988
A_52_P219230 4.8382187
A_51_P207591 5.4656277
A_55_P2131920 7.3177757
A_55_P2404223 6.415245
A_55_P2101944 17.625296
A_52_P358860 7.46255
A_51_P119031 8.469414
A_51_P309854 2.3219483
A_51_P343900 11.160086
A_51_P234359 7.913168
A_51_P487813 8.724604

Total number of rows: 39485

Table truncated, full table size 893 Kbytes.

Supplementary file Size Download File type/resource
GSM3168264_Group5_31.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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