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Sample GSM3168263 Query DataSets for GSM3168263
Status Public on May 30, 2021
Title spleen_low Se_GD_rep1
Sample type RNA
Source name spleen_low Se_GD
Organism Mus musculus
Characteristics strain: balb/c
Sex: female
mouse condition: Graves’ disease (GD)
dietary se supply: low
tissue: spleen
Extracted molecule total RNA
Extraction protocol RNA was isolated from spleen and then quantified by NanoDrop ND-1000 (NanoDrop Technologies Inc., USA). RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Whole Mouse Genome Oligo Microarrays (Agilent Technologies) were used for gene expression analyses and offer a broad view on all known genes and transcripts in the mouse genome.
Label biotin
Label protocol Sample labeling according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Hybridization protocol Array hybridizations were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Scan protocol Agilent Feature Extraction software (version was used to analyze the acquired array images.
Description Group5_10
Data processing Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software package (Agilent Technologies).
Differentially expressed genes between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed using the R scripts. Gene ontology and pathway analyses were performed in the standard enrichment computation method.
Submission date May 31, 2018
Last update date May 30, 2021
Contact name YuFeng Liu
Phone 18691529650
Organization name mouse
Street address The First Affiliated Hospital of Xi'an Jiao Tong University
City Xi'an
State/province ShanXi
ZIP/Postal code 710000
Country China
Platform ID GPL11202
Series (1)
GSE115152 Role of Selenium Intake for Risk and Development of Graves’ Disease

Data table header descriptions
VALUE normalized

Data table
GE_BrightCorner 11.836991
DarkCorner 2.7307217
A_55_P1989846 8.58523
A_55_P1991598 3.8501484
A_55_P2022211 8.625724
A_55_P1980764 8.489102
A_55_P1964375 9.41854
A_51_P128876 13.538272
A_55_P2121042 3.7264223
A_52_P219230 4.5234447
A_51_P207591 5.4842634
A_55_P2131920 7.231541
A_55_P2404223 6.5464435
A_55_P2101944 17.7277
A_52_P358860 7.547439
A_51_P119031 8.399187
A_51_P309854 2.3840065
A_51_P343900 11.11912
A_51_P234359 7.6245875
A_51_P487813 8.002321

Total number of rows: 39485

Table truncated, full table size 894 Kbytes.

Supplementary file Size Download File type/resource
GSM3168263_Group5_10.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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