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Sample GSM3168262 Query DataSets for GSM3168262
Status Public on May 30, 2021
Title spleen_low Se_nonGD_rep3
Sample type RNA
Source name spleen_low Se_nonGD
Organism Mus musculus
Characteristics strain: balb/c
Sex: female
mouse condition: non-GD (Graves’ disease)
dietary se supply: low
tissue: spleen
Extracted molecule total RNA
Extraction protocol RNA was isolated from spleen and then quantified by NanoDrop ND-1000 (NanoDrop Technologies Inc., USA). RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Whole Mouse Genome Oligo Microarrays (Agilent Technologies) were used for gene expression analyses and offer a broad view on all known genes and transcripts in the mouse genome.
Label biotin
Label protocol Sample labeling according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Hybridization protocol Array hybridizations were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology)
Scan protocol Agilent Feature Extraction software (version was used to analyze the acquired array images.
Description Group1_78
Data processing Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software package (Agilent Technologies).
Differentially expressed genes between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed using the R scripts. Gene ontology and pathway analyses were performed in the standard enrichment computation method.
Submission date May 31, 2018
Last update date May 30, 2021
Contact name YuFeng Liu
Phone 18691529650
Organization name mouse
Street address The First Affiliated Hospital of Xi'an Jiao Tong University
City Xi'an
State/province ShanXi
ZIP/Postal code 710000
Country China
Platform ID GPL11202
Series (1)
GSE115152 Role of Selenium Intake for Risk and Development of Graves’ Disease

Data table header descriptions
VALUE normalized

Data table
GE_BrightCorner 11.511842
DarkCorner 2.739682
A_55_P1989846 8.561106
A_55_P1991598 4.074153
A_55_P2022211 8.446523
A_55_P1980764 8.558692
A_55_P1964375 9.394086
A_51_P128876 13.021853
A_55_P2121042 3.6713388
A_52_P219230 5.0507355
A_51_P207591 7.315403
A_55_P2131920 7.1170273
A_55_P2404223 6.410079
A_55_P2101944 17.660387
A_52_P358860 7.317525
A_51_P119031 7.828009
A_51_P309854 2.7969847
A_51_P343900 11.020916
A_51_P234359 6.8652797
A_51_P487813 8.856147

Total number of rows: 39485

Table truncated, full table size 894 Kbytes.

Supplementary file Size Download File type/resource
GSM3168262_Group1_78.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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