|Public on Sep 03, 2008
|Xenopus laevis Brain, biological replicate 3
|Xenopus laevis, Adult male hindbrain (dorsal tegmental area of the medulla to the end of n.IX-X)
|Adult male Xenopus laevis
|Animal was immersed in MS222 and tissue was immediately extracted
|Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
|Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
|Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
|GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|1 of 3 biological replicates of gene expression data from adult brain.
|Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.laevis testis
|Aug 29, 2008
|Last update date
|Dec 03, 2008
|1280 Main Street West
|Expression data for a study on cross-species hybridization on single-species microarrays