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Sample GSM316737 Query DataSets for GSM316737
Status Public on Sep 03, 2008
Title Xenopus laevis Brain, biological replicate 3
Sample type RNA
Source name Xenopus laevis, Adult male hindbrain (dorsal tegmental area of the medulla to the end of n.IX-X)
Organism Xenopus laevis
Characteristics Adult male Xenopus laevis
Treatment protocol Animal was immersed in MS222 and tissue was immediately extracted
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
Hybridization protocol Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description 1 of 3 biological replicates of gene expression data from adult brain.
Data processing Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.laevis testis
Submission date Aug 29, 2008
Last update date Dec 03, 2008
Contact name Frederic Chain
Organization name McMaster University
Department Biology
Lab LSB 324
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S4K1
Country Canada
Platform ID GPL1318
Series (1)
GSE12625 Expression data for a study on cross-species hybridization on single-species microarrays

Data table header descriptions
VALUE RMA normalized (log2) using RMA express using "XB+XL perfect match probemask"

Data table
Xl.1.1.S1_s_at 12.1680276
Xl.10012.1.A1_at 4.373748285
Xl.10032.1.A1_s_at 8.424945503
Xl.10043.1.A1_at 6.164749458
Xl.10043.2.S1_at 3.944353133
Xl.10054.1.S1_at 8.00794894
Xl.10087.1.A1_at 10.64109769
Xl.10104.1.A1_at 7.329673402
Xl.10118.1.S1_at 6.094562645
Xl.1013.1.S1_at 4.020277188
Xl.10142.1.S1_at 7.036227406
Xl.1015.1.S1_at 6.83839061
Xl.10170.1.A1_at 7.346549226
Xl.10191.1.S1_at 7.290864231
Xl.10208.1.S1_at 7.245354461
Xl.10222.1.A1_at 5.338798126
Xl.10227.1.A1_at 8.837187369
Xl.10230.1.A1_at 7.272664447
Xl.10251.1.A1_at 8.772513558
Xl.10287.1.S1_at 10.04454482

Total number of rows: 2143

Table truncated, full table size 59 Kbytes.

Supplementary file Size Download File type/resource
GSM316737.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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