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Sample GSM316733 Query DataSets for GSM316733
Status Public on Sep 03, 2008
Title Xenopus borealis Testis, biological rep 2
Sample type RNA
 
Source name Xenopus borealis, sexually mature testis
Organism Xenopus borealis
Characteristics Adult male Xenopus borealis (Kenya)
Treatment protocol Animal was immersed in MS222 and gonad was immediately extracted
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
 
Hybridization protocol Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description 1 of 3 biological replicates of gene expression data from sexually mature testis.
Data processing Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.borealis brain
 
Submission date Aug 29, 2008
Last update date Sep 02, 2008
Contact name Frederic Chain
E-mail(s) chainfj@mcmaster.ca
Organization name McMaster University
Department Biology
Lab LSB 324
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S4K1
Country Canada
 
Platform ID GPL1318
Series (1)
GSE12625 Expression data for a study on cross-species hybridization on single-species microarrays

Data table header descriptions
ID_REF
VALUE RMA normalized (log2) using RMA express using "XB+XL perfect match probemask"

Data table
ID_REF VALUE
Xl.1.1.S1_s_at 12.91319638
Xl.10012.1.A1_at 7.554967962
Xl.10032.1.A1_s_at 9.291889199
Xl.10043.1.A1_at 6.016640145
Xl.10043.2.S1_at 3.750678852
Xl.10054.1.S1_at 9.172969255
Xl.10087.1.A1_at 9.686748229
Xl.10104.1.A1_at 6.214159318
Xl.10118.1.S1_at 5.575660768
Xl.1013.1.S1_at 6.651889073
Xl.10142.1.S1_at 8.012298651
Xl.1015.1.S1_at 8.80764765
Xl.10170.1.A1_at 7.960967131
Xl.10191.1.S1_at 9.471226757
Xl.10208.1.S1_at 6.583966427
Xl.10222.1.A1_at 5.896546095
Xl.10227.1.A1_at 7.167583612
Xl.10230.1.A1_at 8.232899693
Xl.10251.1.A1_at 5.439727513
Xl.10287.1.S1_at 8.718218291

Total number of rows: 2143

Table truncated, full table size 59 Kbytes.




Supplementary file Size Download File type/resource
GSM316733.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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