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Sample GSM3151764 Query DataSets for GSM3151764
Status Public on May 25, 2018
Title Sample78: Umbilical cord blood - ControlTE
Sample type RNA
 
Source name Umbilical cord blood
Organism Homo sapiens
Characteristics tissue: Umbilical cord blood
maternal diagnosis: ControlTE
neonate gender: Male
rin: 8.9
microarray batch: Two
Treatment protocol Umbilical cord blood was collected by trained staff after offspring delivery but before delivery of the placenta. The cord was clamped and cut, after which the clamp was released and cord blood drained by gravity into a kidney collection dish. Cord blood was collected using a syringe for processing and storage at -80C in PAXgene RNA tubes.
Extracted molecule total RNA
Extraction protocol RNA was extracted from PAXgene RNA tubes as per the manufacturer's instructions. All samples had a Bioanalyzer RNA Integrity Number (RIN) >7.
Label biotin
Label protocol Complementary DNA was derived from RNA and then hybridized, and raw probe intensities were generated on Illumina HumanHT-12 v4 BeadChip arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description rep1
Data processing All statistical analyses were conducted in the statistical package R. All gene expression data were processed, normalized and quality treated. Raw data were background corrected, quantile normalized and log2 ransformed using the neqc function of limma, a robust method for Illumina BeadChips. Non-specific filtering retained probes expressed in at least 50 arrays with a detection P-value <0.05. When multiple microarray probes mapped to the same HGNC symbol, we used a median reduction technique and used the resulting collapsed expression profile for each gene for further analysis. Resulting normalized data were inspected for outlying samples using unsupervised hierarchical clustering of samples and principal component analysis to identify potential outliers outside two standard deviations from these grand averages. Based on these metrics, 19 samples were labeled as outliers and 149 samples passed into the subsequent analyses.
 
Submission date May 24, 2018
Last update date May 25, 2018
Contact name Michael S Breen
Organization name Ichan School of Medicine at Mount Sinai
Department Depts. of Psychiatry, Genetics and Genomic Sciences
Street address 1468 Madison Ave
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL10558
Series (1)
GSE114852 Gene expression in cord blood links genetic risk for neurodevelopmental disorders with maternal psychological distress and adverse childhood outcomes

Data table header descriptions
ID_REF
VALUE The neqc function of limma was used to normalize all data.
SAMPLE78 DetectionPval

Data table
ID_REF VALUE SAMPLE78 DetectionPval
ILMN_1343291 13.1052 0
ILMN_1343295 8.203 0
ILMN_1651229 4.5565 0.0091
ILMN_1651254 6.0524 0
ILMN_1651259 4.5972 0.0078
ILMN_1651262 7.2385 0
ILMN_1651279 4.5482 0.0104
ILMN_1651282 9.2749 0
ILMN_1651288 4.717 0.0065
ILMN_1651315 5.8984 0
ILMN_1651316 5.3669 0.0013
ILMN_1651328 4.5182 0.0117
ILMN_1651346 4.8114 0.0052
ILMN_1651347 6.8836 0
ILMN_1651373 6.2682 0
ILMN_1651378 5.0284 0.0039
ILMN_1651385 5.2369 0.0026
ILMN_1651403 4.5937 0.0078
ILMN_1651405 5.9726 0
ILMN_1651433 7.8562 0

Total number of rows: 13405

Table truncated, full table size 329 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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