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Sample GSM3146403 Query DataSets for GSM3146403
Status Public on Apr 30, 2019
Title H1975_TKI_b
Sample type SRA
 
Source name H1975 Cell line
Organism Homo sapiens
Characteristics treatment: WZ4002
dose: 300nM
cell line: H1975
Treatment protocol Cells were treated with vehicle or the indicated concentrations of Gefitinib/WZ4002 for 2-3 weeks
Growth protocol cells were grown in RPMI supplemented with 10% FBS at 37 degrees C and 5% CO2
Extracted molecule polyA RNA
Extraction protocol Total cellular RNA using Qiagen RNEasy kit
Strand-specific RNA-seq library preparation using the Ovation Universal RNA-Seq System 1-96; For PC9 and MGH119 - Illumina Poly-A kit followed by NEB's Ultra Directional Library Prep
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing RNA-seq reads were aligned to the hg19genome assembly using STAR with default parameter
Counted with htseq-0.6.1 based on UCSC gene annotations
Genome_build: hg19
Supplementary_files_format_and_content: read count abundance measurements were generated using htseq
 
Submission date May 18, 2018
Last update date Apr 30, 2019
Contact name Sana Raoof
E-mail(s) sanaraoofmgh@gmail.com
Organization name Massachusetts General Hospital
Street address 175 Cambridge St
City Boston
ZIP/Postal code 02114
Country USA
 
Platform ID GPL16791
Series (1)
GSE114647 Targeting FGFR overcomes EMT-mediated resistance in EGFR mutant non-small cell lung cancer
Relations
BioSample SAMN09223853
SRA SRX4099673

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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