|
Status |
Public on Apr 30, 2019 |
Title |
H1975_TKI_a |
Sample type |
SRA |
|
|
Source name |
H1975 Cell line
|
Organism |
Homo sapiens |
Characteristics |
treatment: WZ4002 dose: 300nM cell line: H1975
|
Treatment protocol |
Cells were treated with vehicle or the indicated concentrations of Gefitinib/WZ4002 for 2-3 weeks
|
Growth protocol |
cells were grown in RPMI supplemented with 10% FBS at 37 degrees C and 5% CO2
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total cellular RNA using Qiagen RNEasy kit Strand-specific RNA-seq library preparation using the Ovation Universal RNA-Seq System 1-96; For PC9 and MGH119 - Illumina Poly-A kit followed by NEB's Ultra Directional Library Prep
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
RNA-seq reads were aligned to the hg19genome assembly using STAR with default parameter Counted with htseq-0.6.1 based on UCSC gene annotations Genome_build: hg19 Supplementary_files_format_and_content: read count abundance measurements were generated using htseq
|
|
|
Submission date |
May 18, 2018 |
Last update date |
Apr 30, 2019 |
Contact name |
Sana Raoof |
E-mail(s) |
sanaraoofmgh@gmail.com
|
Organization name |
Massachusetts General Hospital
|
Street address |
175 Cambridge St
|
City |
Boston |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE114647 |
Targeting FGFR overcomes EMT-mediated resistance in EGFR mutant non-small cell lung cancer |
|
Relations |
BioSample |
SAMN09223854 |
SRA |
SRX4099672 |