GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM3136794 Query DataSets for GSM3136794
Status Public on Jun 20, 2018
Title BLC_73
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.674856259031
1053_at 7.48883975695488
117_at 6.45158145160475
121_at 7.59701434134879
1255_g_at 3.3177149492268
1294_at 6.60765181332944
1316_at 5.10736166531404
1320_at 5.1783003603694
1405_i_at 9.40132020958549
1431_at 3.54675637001678
1438_at 7.64745322470445
1487_at 7.23593880332616
1494_f_at 5.16931022170182
1552256_a_at 9.62814589362527
1552257_a_at 9.38421597815349
1552258_at 4.91439289977786
1552261_at 4.20653800339359
1552263_at 6.20033849047581
1552264_a_at 8.22525720683367
1552266_at 3.94783450198927

Total number of rows: 54675

Table truncated, full table size 1478 Kbytes.

Supplementary file Size Download File type/resource
GSM3136794_BC04_ARN0065_s1h1s1_U133p2.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap