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Sample GSM3136792 Query DataSets for GSM3136792
Status Public on Jun 20, 2018
Title BLC_71
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 8.92829008035381
1053_at 7.68744402083456
117_at 6.13585962442363
121_at 7.08760395068313
1255_g_at 3.22439462979967
1294_at 6.96633760006543
1316_at 4.93556746122547
1320_at 4.87255265123448
1405_i_at 8.24556351570465
1431_at 3.57385181024252
1438_at 7.55039092848327
1487_at 7.19240037801195
1494_f_at 5.59783209616123
1552256_a_at 7.81344450597722
1552257_a_at 7.75918458912896
1552258_at 4.68283985784862
1552261_at 4.23727734054206
1552263_at 5.7046006850715
1552264_a_at 7.63585108456635
1552266_at 5.43956218889339

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136792_BC04_ARN0060_s1h1s1_U133p2.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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