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Sample GSM3136785 Query DataSets for GSM3136785
Status Public on Jun 20, 2018
Title BLC_63
Sample type RNA
 
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
 
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
E-mail(s) cecile.laurent@curie.fr
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
 
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
ID_REF
VALUE RMA signal intensity.

Data table
ID_REF VALUE
1007_s_at 9.01668036107396
1053_at 8.09551150266664
117_at 8.52904822740948
121_at 7.16391571025792
1255_g_at 3.69210280008689
1294_at 7.80534391873582
1316_at 4.8631048711904
1320_at 4.51831555499835
1405_i_at 9.9761969639195
1431_at 3.64103544826492
1438_at 6.84144149814576
1487_at 7.23820773114516
1494_f_at 5.15887515242789
1552256_a_at 8.72590799864594
1552257_a_at 8.4959706652571
1552258_at 4.78931892845166
1552261_at 4.65092571859998
1552263_at 6.07374962487463
1552264_a_at 7.63216964471875
1552266_at 3.70337806608335

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM3136785_BC04_ARN0043_s1h1s1_U133p2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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