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Sample GSM3136784 Query DataSets for GSM3136784
Status Public on Jun 20, 2018
Title BLC_62
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 9.72888755092636
1053_at 8.92762268932674
117_at 7.53014287012104
121_at 7.06378731756766
1255_g_at 3.05076742729095
1294_at 6.78662925064501
1316_at 4.78513973154295
1320_at 4.83481278611651
1405_i_at 9.55866348180683
1431_at 3.31577440129091
1438_at 8.50274360944139
1487_at 7.20256898616973
1494_f_at 5.15846336743187
1552256_a_at 9.25029182959689
1552257_a_at 8.81970131448998
1552258_at 4.15737406200605
1552261_at 4.33469567545848
1552263_at 7.20796474137045
1552264_a_at 8.79362938124436
1552266_at 3.77048847947836

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136784_BC16_ARN0096_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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