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Sample GSM3136783 Query DataSets for GSM3136783
Status Public on Jun 20, 2018
Title BLC_61
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.0746557799248
1053_at 7.13031520546338
117_at 6.84138215246087
121_at 7.81178505253731
1255_g_at 3.24477871643532
1294_at 7.13583232961038
1316_at 5.2366803595573
1320_at 4.84479891145487
1405_i_at 7.31156503762978
1431_at 3.66793046447641
1438_at 9.4031480428067
1487_at 6.62113369970472
1494_f_at 5.35733166757785
1552256_a_at 8.51923826579474
1552257_a_at 8.66407090686781
1552258_at 4.42171172089098
1552261_at 4.52669310291338
1552263_at 5.88662637150564
1552264_a_at 7.54327033409008
1552266_at 4.11372807886662

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136783_BC16_ARN0102_s1h1s1_U133p2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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