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Sample GSM3136782 Query DataSets for GSM3136782
Status Public on Jun 20, 2018
Title BLC_60
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.586089753072
1053_at 8.39108264430802
117_at 6.982073587858
121_at 7.29982472650552
1255_g_at 3.13040597810882
1294_at 6.32296503045882
1316_at 4.71600679964951
1320_at 4.35239922175833
1405_i_at 6.58777256027648
1431_at 4.06561499819205
1438_at 12.1029032741209
1487_at 8.44695307139213
1494_f_at 5.32146542758231
1552256_a_at 8.83127487620909
1552257_a_at 9.14988355105134
1552258_at 4.43355502307854
1552261_at 4.53691266988157
1552263_at 5.82904746412106
1552264_a_at 7.64163597203463
1552266_at 4.07467454518395

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136782_BC16_ARN0117_s1h1s1_U133p2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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