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Sample GSM3136774 Query DataSets for GSM3136774
Status Public on Jun 20, 2018
Title BLC_50
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.8273641955214
1053_at 6.71274502382361
117_at 6.46474563833696
121_at 6.89331340995206
1255_g_at 3.19764897677973
1294_at 7.78604461311157
1316_at 4.91927220368315
1320_at 4.4124214491686
1405_i_at 9.08080395265531
1431_at 3.64315826562686
1438_at 9.20350357229018
1487_at 7.54899359088514
1494_f_at 5.30315445485753
1552256_a_at 8.70390174761647
1552257_a_at 9.13896242804284
1552258_at 4.80087985259065
1552261_at 4.21482333920699
1552263_at 6.14577568254455
1552264_a_at 6.67693966640644
1552266_at 3.7184074246174

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136774_BC16_ARN0076_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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