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Sample GSM3136773 Query DataSets for GSM3136773
Status Public on Jun 20, 2018
Title BLC_40
Sample type RNA
 
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
 
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
E-mail(s) cecile.laurent@curie.fr
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
 
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
ID_REF
VALUE RMA signal intensity.

Data table
ID_REF VALUE
1007_s_at 9.91524411717617
1053_at 6.88547699163727
117_at 6.78283272245603
121_at 7.69943040162828
1255_g_at 3.36558816357105
1294_at 6.78297893069872
1316_at 4.86178436307169
1320_at 4.48349389315882
1405_i_at 8.96592513915015
1431_at 3.38352660107097
1438_at 8.30031155607493
1487_at 7.64498472790068
1494_f_at 5.40493710297507
1552256_a_at 8.85590715810584
1552257_a_at 8.51472373314972
1552258_at 4.67818511108446
1552261_at 4.16503066538412
1552263_at 5.96411969671804
1552264_a_at 8.2815575628405
1552266_at 3.51384226070827

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM3136773_BC16_ARN0069_s1h1s1_U133p2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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