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Sample GSM3136772 Query DataSets for GSM3136772
Status Public on Jun 20, 2018
Title BLC_39
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 9.13911305538102
1053_at 8.05646492437371
117_at 7.62403947188306
121_at 7.04875489384089
1255_g_at 3.08950911160343
1294_at 7.87897828926025
1316_at 5.17057395462692
1320_at 4.41033881964926
1405_i_at 10.0738810002961
1431_at 3.35367321370318
1438_at 7.07304980404797
1487_at 7.18106833626902
1494_f_at 4.97116283672956
1552256_a_at 9.15751148119165
1552257_a_at 8.52942063688443
1552258_at 4.49886379851708
1552261_at 4.73529955098124
1552263_at 5.90312619228607
1552264_a_at 7.91161756336045
1552266_at 3.8337402570647

Total number of rows: 54675

Table truncated, full table size 1478 Kbytes.

Supplementary file Size Download File type/resource
GSM3136772_BC04_ARN0019_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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