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Sample GSM3136769 Query DataSets for GSM3136769
Status Public on Jun 20, 2018
Title BLC_36
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.1767893240668
1053_at 7.95688427975883
117_at 6.93697545153566
121_at 6.89975239144104
1255_g_at 3.18339046896899
1294_at 7.62381850829149
1316_at 4.86678763249963
1320_at 4.90704428347448
1405_i_at 9.13793118823639
1431_at 3.54542972753602
1438_at 8.96553343915596
1487_at 6.63578464992703
1494_f_at 5.0559636253233
1552256_a_at 9.07659756464756
1552257_a_at 8.35585156038792
1552258_at 4.8670860281432
1552261_at 4.61252824047907
1552263_at 6.2196053132057
1552264_a_at 7.89695219350101
1552266_at 3.65098634680316

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136769_BC04_ARN0011_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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