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Sample GSM3136765 Query DataSets for GSM3136765
Status Public on Jun 20, 2018
Title BLC_31
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: non medullary basal-like breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.4072834848885
1053_at 9.01157952020237
117_at 5.86570368700901
121_at 7.88376952866033
1255_g_at 3.454631817639
1294_at 6.8692300338839
1316_at 5.12828248598288
1320_at 4.3696895360038
1405_i_at 10.5030030863092
1431_at 3.66211006387125
1438_at 7.38565731592577
1487_at 7.5420755315432
1494_f_at 5.69452742564283
1552256_a_at 9.55463106834671
1552257_a_at 9.386662769354
1552258_at 4.5006407779401
1552261_at 4.6780471323578
1552263_at 4.66054468685193
1552264_a_at 8.12530175874275
1552266_at 3.50821825191498

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136765_BC16_ARN0110_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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