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Sample GSM3136763 Query DataSets for GSM3136763
Status Public on Jun 20, 2018
Title MBC43
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 9.65745510126368
1053_at 7.97332620324102
117_at 7.33550508837569
121_at 7.75570868347435
1255_g_at 3.1776552665261
1294_at 8.52365274971504
1316_at 4.80297450328026
1320_at 4.34026088447114
1405_i_at 12.0493491887312
1431_at 3.59799776288865
1438_at 7.32770894336688
1487_at 7.59484271704807
1494_f_at 5.05024245066276
1552256_a_at 8.82802717983661
1552257_a_at 8.63892391884766
1552258_at 4.55042916514936
1552261_at 4.71649267287292
1552263_at 6.47267183431273
1552264_a_at 8.20168577745049
1552266_at 3.4552878234578

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136763_TM01_ARN0026_s1h1s1_U133p2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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