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Sample GSM3136762 Query DataSets for GSM3136762
Status Public on Jun 20, 2018
Title MBC44
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.1407196778645
1053_at 7.59247177698673
117_at 7.25485297531317
121_at 8.12725944104814
1255_g_at 3.32678881237697
1294_at 7.59540437492146
1316_at 4.95937070988327
1320_at 4.59574145175189
1405_i_at 9.11976740773559
1431_at 3.66752802383118
1438_at 8.39010236440111
1487_at 7.3745672065635
1494_f_at 5.21636615521661
1552256_a_at 9.70238260704257
1552257_a_at 7.93733581719315
1552258_at 4.80674461807588
1552261_at 5.56462603567027
1552263_at 6.5908506189473
1552264_a_at 8.72360799113192
1552266_at 3.99449002713283

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136762_TM01_ARN0025_s1h1s1_U133p2.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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