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Sample GSM3136755 Query DataSets for GSM3136755
Status Public on Jun 20, 2018
Title MBC03
Sample type RNA
 
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
 
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
E-mail(s) cecile.laurent@curie.fr
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
 
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
ID_REF
VALUE RMA signal intensity.

Data table
ID_REF VALUE
1007_s_at 9.96115986915085
1053_at 8.4426923994004
117_at 6.9177048912615
121_at 7.37503284457495
1255_g_at 3.25294200109226
1294_at 8.1654702144265
1316_at 4.83076404943153
1320_at 4.41515592220733
1405_i_at 11.3422601936944
1431_at 3.41327810603014
1438_at 6.85744913034704
1487_at 7.03197612101089
1494_f_at 5.07040388215098
1552256_a_at 9.72285972840308
1552257_a_at 8.96972664378442
1552258_at 4.50460829716876
1552261_at 5.58245316465461
1552263_at 6.56323678066651
1552264_a_at 8.02166326440668
1552266_at 3.70927001109648

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM3136755_TM01_ARN0017_s1h1s1_U133p2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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