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Sample GSM3136753 Query DataSets for GSM3136753
Status Public on Jun 20, 2018
Title MBC05
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.5591182427655
1053_at 7.87919688247429
117_at 6.87937217110539
121_at 7.6622091675848
1255_g_at 3.04886383740899
1294_at 7.84406127344499
1316_at 4.925413351173
1320_at 4.63483631085205
1405_i_at 9.16966148412456
1431_at 3.57839172654821
1438_at 7.91809070500139
1487_at 7.44132676573025
1494_f_at 5.29102771639661
1552256_a_at 8.38796226949403
1552257_a_at 9.02142632235569
1552258_at 4.56774124327099
1552261_at 4.47304312310316
1552263_at 5.92104866101981
1552264_a_at 7.34872913843716
1552266_at 3.87574192239873

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136753_TM01_ARN0015_s1h1s1_U133p2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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